Comparison of the effect of calcium(II) and manganese(II) ions on trypsin autolysis |
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Authors: | Tamás Vajda Attila Garai |
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Institution: | Institute of Organic Chemistry, L. Eötvös University, Múzeum körut 4/B, Budapest Hungary |
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Abstract: | The effect of Mn2+ and Ca2+ ions on the rate of trypsin autolysis was studied at pH 7.0 and at 34.4-60.2°C. For comparison, the kinetic constants of esterolytic activity of trypsin in the presence of the metal ion were determined at pH 7.4 and at 36° and 40°C. There was no significant difference in the rate of autolysis between Mn2+ and Ca2+ in the temperature range 34-47°C, but at 56.8° and 60.2° autolysis was slightly more rapid in the presence of Mn2+. The Mn2+ or Ca2+ ion bound to trypsin is supposed to control the conformation and thereby the stability and the activity of the enzyme. This indirect effect of Mn2+ and Ca2+ is discussed on a structural basis of the enzyme molecule. |
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