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| 基质金属蛋白酶-3和血管内皮生长因子在不同月龄大鼠椎间盘组织中的表达及意义 | |
| 引用本文： | 贾长青,王臣,陈勇,梁峰,王青.基质金属蛋白酶-3和血管内皮生长因子在不同月龄大鼠椎间盘组织中的表达及意义[J].中国组织化学与细胞化学杂志,2006,15(3):355-360. |
| 作者姓名： | 贾长青 王臣 陈勇 梁峰 王青 |
| 作者单位： | 1. 中国医科大学附属第二医院骨外科,沈阳,110004 2. 沈阳市第四人民医院骨外科,沈阳,110031 3. 沈阳市120急救中心,沈阳,110006 4. 抚顺矿物局总医院,抚顺,113008 |
| 摘 要： | 目的检测不同月龄大鼠的椎间盘组织中基质金属蛋白酶-3(MMP3)和血管内皮生长因子(VEGF)的表达,探讨MMP3和VEGF与椎间盘自发退变的关系。方法采用苏木精-伊红(HE)染色和链酶亲和素-过氧化物酶复合物(SABC)免疫组化方法及显微图像分析技术,测定50只Wistar大鼠(1、3、6、12、18月龄各10只)椎间盘组织中MMP3和VEGF的表达情况并观察椎间盘组织学变化。结果随着大鼠月龄的增长,其椎间盘中MMP3和VEGF的表达亦发生变化,由低月龄组(1-3月龄)至成龄组(12月龄)MMP3表达逐渐增多,而VEGF表达逐渐下降,且这种变化有显著统计学意义(P<0.01)。椎间盘的组织结构也发生变化,随月龄增加,椎间盘中软骨细胞数量减少,胶原纤维增生、粗大、走行紊乱。结论大鼠椎间盘结构随月龄增长发生性改变,椎间盘中MMP3和VEGF表达发生变化,这可能引起椎间盘的自发退变,而且这种改变与人的椎间盘的自然退变具有相似性和可比拟性。 |
| 关 键 词： | 基质金属蛋白酶-3 血管内皮生长因子 椎间盘 |
| 收稿时间： | 10 19 2005 12:00AM |
| 修稿时间： | 02 3 2006 12:00AM |
THE EXPRESSION AND SIGNIFICANCE OF MATRIX METALLOPROTEINASE-3 AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN THE INTERVERTEBRAL DISC OF RATS OF DIFFERENI AGES |
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| Jia Changqing,Wang Chen,Chen Yong,Liang Feng,Wang Qing.THE EXPRESSION AND SIGNIFICANCE OF MATRIX METALLOPROTEINASE-3 AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN THE INTERVERTEBRAL DISC OF RATS OF DIFFERENI AGES[J].Chinese Journal of Histochemistry and Cytochemistry,2006,15(3):355-360. | |
| Authors: | Jia Changqing Wang Chen Chen Yong Liang Feng Wang Qing |
| Institution: | Department of Orthopaedic Surgery, No. 2 Affiliated Hospital of China Medical University, Shengang 110004 ; 1 Department of Orthopaedic Surgery, No. 4 Peoples Hospital, Shenyang 110031; 2 Department of Orthopaedic Surgery, 120 First Aid Center, Shenyang 110006; 3 General Hospital of Fushun Bureau of Mines, Fushun 113008, China |
| Abstract: | Objective To determine the expression of matrix metalloproteinase3(MMP-3) and vascular endothelial growth factor(VEGF) in the intervertebral disc tissue of Wistar rats of different ages in order to provide insights into the pathogenesis of retrogressive metamorphosis of annulus fibrosus.Methods The histological changes of intervertebral disc tissuewas observed by haematoxyllineosin staining,and the presence of MMP_(3 )and VEGF inintervertebral disctissue of 50 Wistar rats(10 each in groups of 1,3,6,12 and 18 months) were determined by SABC immunohistochemical staining and the micrography analysis system.Results The MMP-3-and VEGF-positive cell ratios significantly varied with aging.The histological structure of intervertebral disc tissuechanged,the number of chondrocytes decreased,and the collagens were thick,hyperplastic and disorganized.Conclusion The lopsided expression of MMP_(3)and VEGFmay be related with the retrogressive metamorphosisof the intervertebral disc and play an important role in intervertebral disc herniation. |
| Keywords: | Matrix metalloproteinase-3 Vascular endothelial growth factor Intervertebral disc |
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