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Isolation and characterization of microsatellite markers for <Emphasis Type="Italic">Cedrela odorata</Emphasis> L. (Meliaceae), a high value neotropical tree
Authors:G Hernández  A Buonamici  K Walker  G G Vendramin  C Navarro  S Cavers
Institution:1.Centro Agrónomico Tropical de Investigación y Ense?anza,Turrialba,Costa Rica;2.NERC Centre for Ecology and Hydrology, CEH Edinburgh,Midlothian,Scotland, UK;3.Plant Genetics Institute,Consiglio Nazionale delle Ricerche (CNR),Sesto Fiorentino,Italy
Abstract:We describe 9 primers for amplification of microsatellite loci for the Neotropical tree Cedrela odorata L. (Meliaceae). Loci were isolated from an enriched library derived from a single DNA sample from a tree in Costa Rica. Levels of polymorphism were determined using samples from a large progeny trial. Across loci, the number of alleles ranged from 14 to 30. Observed heterozygosity levels ranged from 0.61 to 0.88. No linkage disequilibria were detected although some departures from Hardy-Weinberg equilibrium (HWE) were found, probably due to a Wahlund effect.
Keywords:Cedrela           odorata            Cedar  Meliaceae  Microsatellites
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