Isolation and characterization of microsatellite markers for <Emphasis Type="Italic">Cedrela odorata</Emphasis> L. (Meliaceae), a high value neotropical tree |
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Authors: | G Hernández A Buonamici K Walker G G Vendramin C Navarro S Cavers |
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Institution: | 1.Centro Agrónomico Tropical de Investigación y Ense?anza,Turrialba,Costa Rica;2.NERC Centre for Ecology and Hydrology, CEH Edinburgh,Midlothian,Scotland, UK;3.Plant Genetics Institute,Consiglio Nazionale delle Ricerche (CNR),Sesto Fiorentino,Italy |
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Abstract: | We describe 9 primers for amplification of microsatellite loci for the Neotropical tree Cedrela odorata L. (Meliaceae). Loci were isolated from an enriched library derived from a single DNA sample from a tree in Costa Rica. Levels
of polymorphism were determined using samples from a large progeny trial. Across loci, the number of alleles ranged from 14
to 30. Observed heterozygosity levels ranged from 0.61 to 0.88. No linkage disequilibria were detected although some departures
from Hardy-Weinberg equilibrium (HWE) were found, probably due to a Wahlund effect. |
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Keywords: | Cedrela odorata Cedar Meliaceae Microsatellites |
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