汉滩病毒毒力标志的建立

ESTABLISHMENT OF MARKERS FOR VIRULENCEOF HANTAAN VIRUS

ell culture prolifeation, ability for inducing neutralizing antibodies and McAb raictionprofile were compatal between the attenuated clone A39s and the virulent clone A9v ofHantaan virus isolate A9. Differences were found : the attenuated virus clone A39s lost itsinfectivity to peritoneal macrophages of BAlB/c mice and human peripheral bloodmononuclear leukocytes;A39s virus grew slowly on Vero-E6 cells, a longer time was neededto form plaques; and size of the plaques formed was much smaller than those of A9v virus.No significant alternations were observed in cell fusion function, stimulating cytotoxic cellactivity, the adsorption and penetration rate on Vero-E6 cell culture between A39s virusand A9v virus. It was demonstrated that A39s virus was not temperature-sensitive(ts)and contained no defective interfering particles(DIP). No substantial differences in antibodyresponse to these two viruses were observed, A39s virus could induce high titered neutralizingantibody and could cross-neutralize some other strains of Hantaan virus. Furthermore,theMcAb reaction profiles by indirect IFA and PRNT between these two viruses were compared.It was found that both had the same reactivity with 18 anti-nucleoprotein(NP)McAbsand 18 anti-glycoprotein(G)McAbs except 4 anti-G McAbs, 3D5, 6D4, 16DZ and 8F8which suggested that some genetic variations might have occurred on the glycoprotein of A39svirus, especially on the Gl glycoprotein.