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铁皮石斛疫病及其病原菌
引用本文:李静,张敬泽,吴晓鹏,单卫星,徐同.铁皮石斛疫病及其病原菌[J].菌物学报,2008,27(2):171-176.
作者姓名:李静  张敬泽  吴晓鹏  单卫星  徐同
作者单位:1. 浙江大学生物技术研究所,杭州,310029
2. 西北农林科技大学植物保护学院,杨凌,712100
基金项目:国家科技攻关项目 , 浙江省自然科学基金
摘    要:铁皮石斛疫病2001年发现在浙江义乌栽培田间,是由疫霉菌引起的。在田间,疫霉菌侵染茎基部,引起当年移植苗根腐、植株枯萎和死亡,但侵染2-3年植株幼嫩顶部仅引起顶枯症状。通过对病原菌形态学、交配型的观察,以及核糖体DNAITS序列分析,侵染铁皮石斛的5个分离菌株被鉴定为烟草疫霉菌Phytophthora nicotianae。致病性试验表明,铁皮石斛是烟草疫霉菌的寄主。

关 键 词:卵菌  鉴定  烟草疫霉菌
修稿时间:2007年5月10日

The causal agent of Dendrobium candidum blight disease
Authors:LI Jing  ZHANG Jing-Ze  WU Xiao-Peng  SHAN Wei-Xing and XU Tong
Institution:Biotechnology Institute, Zhejiang University, Hangzhou 310029, China;Biotechnology Institute, Zhejiang University, Hangzhou 310029, China;Biotechnology Institute, Zhejiang University, Hangzhou 310029, China;College of Plant Protection, Northwest Agriculture & Forestry University, Yangling 712100, China;Biotechnology Institute, Zhejiang University, Hangzhou 310029, China
Abstract:Dendrobium candidum blight caused by Phytophthora species was found for the first time in cultivated field in Yiwu city, Zhejiang province of China in 2001. One-year-old infected transplanting seedlings demonstrate typical symptoms of Phytophthora root rot, wilting and death,whereas 2-to 3-year-old infected plants only have die-back symptoms with almost no root and basal stem rot. Morphological observation, mating types and DNA sequences analyses proved that five isolates of the causal agent were all P. nicotianae. Pathogenicity tests confirmed that D. candidum is a natural host of P. nicotianae.
Keywords:Oomycota  identification  Phytophthora nicotianae
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