Expansion of human bone marrow-derived mesenchymal stromal cells: serum-reduced medium is better than conventional medium |
| |
Authors: | Katrin Montzka Tobias Führmann Michael Wöltje Gary A Brook |
| |
Institution: | 1. Research Center, Asahi Glass Co. Ltd., 1150 Hazawa-cho, Yokohama 221-8755, Japan;2. University College London, Gower Street, London W1E6BT, UK;3. IIS, University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan;4. RWTH Aachen University, 52064 Aachen, Germany;5. Institut de Physique du Globe de Paris, 1 rue Jussieu, 75005 Paris, France |
| |
Abstract: | Background aimsHuman mesenchymal stromal cells (hMSC) are of enormous interest for various clinical applications. For the expansion of isolated hMSC to relevant numbers for clinical applications, 10% fetal bovine serum (FBS)-supplemented medium is commonly used. The main critical disadvantage of FBS is the possibility of transmission of infectious agents as well as the possibility of immune rejection of the transplanted cells in response to the bovine serum. Therefore, we tested a commercially available medium, Panserin 401, that was specifically developed for serum-free cell cultivation.MethodshMSC were isolated from bone marrow (BM) and expanded in either Dulbecco's modified Eagle medium (DMEM) or Panserin 401 alone, or combined with FBS (2% or 10%), with or without supplementary growth factors. Cell proliferation and cytotoxicity were monitored twice a week for 3 weeks.Results and ConclusionsNo proliferation was observed in any of the serum-free media. However, DMEM/10% FBS (the conventional culture medium for hMSC) and DMEM/2% FBS with growth factors revealed moderate proliferation. Interestingly, the best proliferation was obtained using Panserin 401 supplemented with 2% FBS and growth factors (as well as with 10% FBS). Analysis of cell growth in Panserin 401 supplemented with 2% FBS only or with growth factors only revealed no proliferation, demonstrating the necessity of the combination of 2% FBS and growth factors. Efficient isolation and expansion of hMSC from cancellous bone could also be performed using Panserin 401 with 2% FBS and growth factors. Furthermore, these isolated cultures maintained multipotency, as demonstrated by adipogenic and osteogenic differentiation. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|