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Plasmid-Borne Type E Neurotoxin Gene Clusters in Clostridium botulinum Strains
Authors:Zhen Zhang  Hannamari Hintsa  Ying Chen  Hannu Korkeala  Miia Lindstr?m
Affiliation:Department of Food Hygiene and Environmental Health, Centre of Excellence in Microbial Food Safety Research, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
Abstract:A collection of 36 Clostridium botulinum type E strains was examined by pulsed-field gel electrophoresis (PFGE) and Southern hybridization with probes targeted to botE and orfX1 in the neurotoxin gene cluster. Three strains were found to contain neurotoxin subtype E1 gene clusters in large plasmids of about 146 kb in size.
Keywords:
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