Preparation of validoxylamine A by biotransformation of validamycin A using resting cells of a recombinant Escherichia coli |
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| Authors: | Yu Yin Weiyi Huang Daijie Chen |
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| Affiliation: | (1) Department of Microbiology, Nanjing Agricultural University, Nanjing, P.R. China;(2) Health Creation Centre for Biopharmaceuticals R&D, Shanghai, P.R. China;(3) Shanghai Institute of Pharmaceutical Industry, Shanghai, P.R. China |
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| Abstract: | A gene encoding β-glucosidase was cloned and over-expressed in Escherichia coli. Validamycin A was then biotransformed into validoxylamine A by using the resting recombinant cells. The biotransformation yield reached 92% when the reaction was performed at 37°C for 1 h in the presence of 100 ml sodium phosphate buffer (0.1 M, pH 7.0), 32 mM validamycin A and 0.71 mg dry cell w/ml. |
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| Keywords: | Biotransformation Recombinant cells Validamycin A Validoxylamine A |
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