Identification of cross-linked peptides from large sequence databases |
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| Authors: | Rinner Oliver Seebacher Jan Walzthoeni Thomas Mueller Lukas N Beck Martin Schmidt Alexander Mueller Markus Aebersold Ruedi |
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| Institution: | Institute of Molecular Systems Biology, Eidgen?ssische Technische Hochschule (ETH) Zurich, Wolfgang-Pauli Strasse 16, 8093 Zurich, Switzerland. |
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| Abstract: | We describe a method to identify cross-linked peptides from complex samples and large protein sequence databases by combining isotopically tagged cross-linkers, chromatographic enrichment, targeted proteomics and a new search engine called xQuest. This software reduces the search space by an upstream candidate-peptide search before the recombination step. We showed that xQuest can identify cross-linked peptides from a total Escherichia coli lysate with an unrestricted database search. |
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