毛竹实时荧光定量PCR内参基因的筛选及成花基因PheTFL1表达分析

通过qRT-PCR对毛竹相关成花基因PheTFL1的表达进行研究,为毛竹开花机理的研究提供理论依据.从毛竹UBC18、PP2A和EF1α等9个候选内参基因中筛选出在叶、幼嫩花序、花序轴、枝、竹青等11个组织器官中都稳定表达的PP2A用于毛竹PheTFL1基因qRT-PCR结果的校正.结果显示:PheTFL1基因在开花竹叶、枝和竹青中低丰度表达,与未开花竹差异不显著,但在花和花序轴中高丰度表达;在实生苗叶和根中高丰度表达,在实生苗茎中低丰度表达.PheTFL1基因在具有分生能力的幼嫩组织中高丰度表达,说明其不仅参与花发育的调控,还参与了分生组织生长的调控.

Screening of Reference Genes Used in qRT-PCR and Expression Analysis of PheTFL1 Gene in Moso Bamboo

The fruits at different developmental stages of Calanthe tsoongiana were sectioned and observed through conventional paraffin section,semithin section and scanning electron microscope.The results showed that:(1)The ovule primordia began to differentiate from placenta 19 days after pollination.The ovule primordia was a layer of epidermal cells encircled a list of cells.The top cell differentiated into archesporial cell.Then archesporial cell differentiated directly into megaspore mother cell,45 days after pollination.(2)The mature ovule was anatropous,bi-integument and tenuinucellar.Development of embryo sac was polygonum type.Ovule development was not synchronized even in the same fruit.(3)The first zygote division was unequal,producing a basal cell and a terminal cell.The basal cell gave rise to the suspensor and it did not participate in the formation of embryo proper.The suspensor degenerated at the late stage of the embryo development.Terminal cell gave rise to embryo proper.Development of embryo was Caryophyllad type.(4)The shape of mature seed liked spindle.It consisted of globular embryo,endopleura and episperm.Endopleura and episperm developed from inner tegument and outer integument respectively.