| H5N1亚型禽流感病毒NS1基因的克隆及其诱导肺腺癌细胞A549凋亡的研究 |
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| 引用本文： | 张传福,蒋世卫,朱恒奇,杨予涛,杨志新,许龙,赵莉霞,周晓巍,黄培堂.H5N1亚型禽流感病毒NS1基因的克隆及其诱导肺腺癌细胞A549凋亡的研究[J].病毒学报,2007,23(5):360-365. |
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| 作者姓名： | 张传福 蒋世卫 朱恒奇 杨予涛 杨志新 许龙 赵莉霞 周晓巍 黄培堂 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 基金项目： | 国家科技支撑计划(2006BAD06A01) |
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| 摘 要： | 通过RT-PCR的方法克隆H5N1亚型禽流感病毒NS1基因,并构建了真核表达载体pCMV-Myc/NS1。将此真核表达质粒转染肺腺癌细胞A549,48 h后,经Western印迹检测,NS1基因能在细胞中正确表达。经荧光显微镜、透射电镜观察和流式细胞仪检测,发现该株流感病毒的NS1蛋白可诱导肺腺癌细胞A549凋亡。
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| 关 键 词： | 禽流感病毒 H5N1亚型 NS1基因 肺腺癌细胞A549 凋亡 |
| 文章编号： | 1000-8721（2007）05-0360-06 |
| 修稿时间： | 2007-03-05 |
Cloning NS1 Gene of H5N1 Avian Influenza Virus and Apoptosis Induced by It in Human Pulmonary Carcinoma Cell Line A549 |
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| ZHANG Chuan-fu,JIANG Shi-wei,ZHU Heng-qi,YANG Yu-tao,YANG Zhi-xin,XU Long,ZHAO Li-xia,ZHOU Xiao-wei,HUANG Pei-tang.Cloning NS1 Gene of H5N1 Avian Influenza Virus and Apoptosis Induced by It in Human Pulmonary Carcinoma Cell Line A549[J].Chinese Journal of Virology,2007,23(5):360-365. |
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| Authors: | ZHANG Chuan-fu JIANG Shi-wei ZHU Heng-qi YANG Yu-tao YANG Zhi-xin XU Long ZHAO Li-xia ZHOU Xiao-wei HUANG Pei-tang |
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| Institution: | Institute of Biotechnology ,Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract: | The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR,and the amplified product was cloned into the eukaryotic expression vector pCMV-Myc,then it was transfected into A549 cells.After 48 h,the expression of NS1 was detected by Western blot.Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apoptosis in human pulmonary carcinoma cell line A549. |
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| Keywords: | avian influenza virus(AIV) H5N1 subtype NS1 gene human pulmonary carcinoma A549 cell line apoptosis |
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