Dark and light green tissues of tobacco leaves systemically infected with tobacco mosaic virus |
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| Authors: | O L Burundukova M V Sapotsky A V Kochetov E A Trifonova V I Malinovsky |
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| Institution: | (1) Institute of Biology and Soil Science, 690022 Vladivostok, Russia;(2) Institute of Cytology and Genetics, 630090 Novosibirsk, Russia |
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| Abstract: | There are significant changes in the structure of the upper tobacco (Nicotiana tabacum L.) leaves systemically infected with tobacco mosaic virus (TMV) especially in the light green tissue (LGT). Dark green areas
(DGI) had intermediate status between healthy tissue and LGT. DGI contained significantly less infectious TMV and viral antigen
than the LGT. The DGI, LGT and healthy tissues did not differ in the permeability of cell membranes and in the set of acidic
pathogenesis-related (PR) proteins but the total content of PR-proteins in the healthy plants was higher than in the infected
ones with the DGI being intermediate between healthy tissue and LGT. The crude leaf extracts from DGI and LGT showed less
total ribonuclease activity and ribonuclease isozymes in comparison with control. |
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| Keywords: | Additional" target="_blank">Additional Nicotiana tabacum pathogenesis-related proteins permeability of cell membranes ribonuclease |
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