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Mitochondrial DNA analysis of Exophiala jeanselmei and Exophiala dermatitidis
Authors:Masako Kawasaki  Hiroshi Ishizaki  Kazuko Nishimura  Makoto Miyaji
Affiliation:(1) Department of Dermatology, Kanazawa Medical University, Uchinada, 920-02 Ishikawa, Japan;(2) Research Center for Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, 280 Chiba, Japan
Abstract:Mitochondrial DNA (mtDNA) analysis with Hae III, Hind. III and Msp I was performed in 45 Exophiala jeanselmei strains (30 Phialophora jeanselmei and 15 Phialophora gougerotii strains) and 31 Exophiala dermatitidis strains. The results were as follows, 1) P. jeanselmei and P. gougerotii are identical, 2) E. jeanselmei is classified into 18 types based on restriction profiles, 3) two strains of E. jeanselmei CBS 577.76 and CBS 578.76 are identified as E. dermatitidis, 4) E. dermatitidis has no intraspecific variation and is definitely distinct from E. jeanselmei, 5) E. jeanselmei is suggested to be a complex organism because of extensive mtDNA polymorphism.
Keywords:DNA  Exophiala dermatitidis  Exophiala gougerotii  Exophiala jeanselmei  mitochondria  restriction profiles  taxonomy
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