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Characterization of recombinant scFv antibody reactive with an apical antigen of Eimeria acervulina |
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| Authors: | Wongi Min Jin-Kyoo Kim Hyun Soon Lillehoj Eun Jung Sohn Jae Yong Han Ki Duk Song Erik Peter Lillehoj |
| Institution: | (1) Department of Microbiology, Changwon National University, Changwon, Korea;(2) U.S. Department of Agriculture, Parasite Biology, Epidemiology and Systematic Laboratory, Animal and Natural Resources Institute, Beltsville, MD, USA;(3) School of Agriculture Biotechnology, Seoul National University, Suwon, 441-744, Korea;(4) Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA |
| Abstract: | The chicken monoclonal antibody (mAb), 6D-12-G10, reacts with an apical complex protein at the anterior tip of E. acervulina sporozoites that inhibits parasite invasion in vitro. Because this mAb was produced at low amount from the original hybridoma cells, an scFv antibody was constructed by amplification of the corresponding V H and V L genes and expressed in E. coli. The scFv antibody was produced at a minimum of 7 mg l–1 and exhibited virtually identical antigen reactivity as the original mAb. |
| Keywords: | apical antigen chicken Eimeria recombinant antibody scFv |
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