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Involvement of colchicine binding site of tubulin in the polymerisation process |
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| Authors: | D Dasgupta R Rajgopalan S Gurnani |
| Affiliation: | Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Bombay 400085, India |
| Abstract: | Role of lysine residues in the colchicine binding site and in the assembly-disassembly process was examined. It was observed that at 4 degrees C (pH 7.5-8, 8 +/- 1) lysine residues and the N-terminal methionine residue of tubulin were all buried within the molecule. Evidence indicates that epsilon-amino groups of lysine residues of tubulin are shared by both the colchicine binding site and the polymerisation process. |
| Keywords: | Tubulin Acetylation Lysine Colchicine binding Polymerisation Conformation MES EGTA GTP guanosine-5′-triphosphate PMG buffer phosphate, magnesium chloride and GTP buffer |
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