Presence of immunoreactive endothelin in human plasma |
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| Authors: | K Ando Y Hirata M Shichiri T Emori F Marumo |
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| Institution: | Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan. |
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| Abstract: | A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of plasma IR-hET was 0.6 fmol/ml. Reverse-phase HPLC coupled with radioimmunoassay revealed two major IR-hET components, one corresponding to authentic hET(1-21) and another with more hydrophilicity than hET(1-21). These data indicate that ET is a circulating vasoconstrictor hormone in man. |
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