| 劳盆地深海热液喷口沉积物中细菌多样性研究 |
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| 引用本文： | 魏曼曼,王玉光,郑 甲,周洪波.劳盆地深海热液喷口沉积物中细菌多样性研究[J].微生物学通报,2009,36(4):0538-0543. |
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| 作者姓名： | 魏曼曼 王玉光 郑 甲 周洪波 |
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| 作者单位： | 1. 中南大学资源加工与生物工程学院,湖南,长沙,410083
2. 中南大学资源加工与生物工程学院,湖南,长沙,410083;中南大学生物冶金教育部重点实验室,湖南,长沙,410083 |
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| 基金项目： | 国家自然科学基金(No. 40646029); 教育部新世纪优秀人才支持计划(No. NECT-06-0691); 国际海底区域研究开发“十一五”项目(No. DYXM-115-02-2-07); 海洋公益性行业科研专项经费项目资助(No. 200805032) |
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| 摘 要： | 采用PCR-RFLP技术调查了劳盆地深海热液喷口两位点沉积物中的细菌多样性。结果表明, 在劳盆地深海热液喷口沉积物环境中细菌多样性十分丰富, 样品DY1中发现6个细菌类群, DY2中则存在4个细菌类群, 其中Gammaproteobacteria细菌亚群和Epsilonproteobacteria细菌亚群在两文库中均占据最大比例, 为沉积物样品中的优势菌群。另外, 在克隆文库中还发现了一些与数据库中的已知序列同源性较低的类群, 从而说明劳盆地深海热液喷口沉积物中存在特有的微生物种属。
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| 关 键 词： | 细菌多样性 劳盆地深海热液喷口 PCR-RFLP 系统发育分析 |
The Diversity of Bacteria in Sediments from Lau Basin Deep-sea Hydrothermal Vents |
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| WEI Man-Man,WANG Yu-Guang,ZHENG Jia and ZHOU Hong-Bo.The Diversity of Bacteria in Sediments from Lau Basin Deep-sea Hydrothermal Vents[J].Microbiology,2009,36(4):0538-0543. |
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| Authors: | WEI Man-Man WANG Yu-Guang ZHENG Jia and ZHOU Hong-Bo |
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| Institution: | School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China;School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China;School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China;(1. School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan 410083, China) (2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha, Hunan 410083, China) |
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| Abstract: | The bacterial diversity of two deep-sea sediments collected at Lau Basin hydrothermal vents were analysed by performing a restriction fragment length polymorphism (RFLP) analysis of 16S rRNA gene sequences. Analysis of sequences showed that there were a diversity of bacterial populations in sediments from Lau Basin deep-sea hydrothermal vents, and six bacterial divisions and four bacterial divisions represented in the sample DY1 and DY2 respectively. The phyla Gammaproteobacteria and Epsilonproteobacteria were the most dominant groups in both clone library DY1 and DY2. Additionally, some bacterial sequences had lower similarity to most known sequences in the database, which suggested that there were some novel taxa of bacteria in sediment from Lau Basin deep-sea hydrothermal vents. |
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| Keywords: | Bacterial diversity Lau Basin deep-sea hydrothermal vent PCR-RFLP Phylogenetic analysis |
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