| 重组人睫状神经营养因子衍生物的构建、表达和活性分析 |
| |
| 引用本文： | 张振龙,徐静,郭秀梅,孙成金,王涛,郭鹏.重组人睫状神经营养因子衍生物的构建、表达和活性分析[J].微生物学免疫学进展,2006,34(4):10-13. |
| |
| 作者姓名： | 张振龙 徐静 郭秀梅 孙成金 王涛 郭鹏 |
| |
| 作者单位： | 北京生物制品研究所,北京,100024 |
| |
| 摘 要： | 采用PCR定点突变技术对睫状神经营养因子(CNTF)基因进行改造,获得了CNTF衍生物(CNTF-D)基因序列分析证明其核苷酸序列符合设计要求,并在大肠杆菌中获得高效表达,CNTF-D表达量超过40%,复性后,经Q-Sepharose HP纯化,其纯度超过95%,体内法测定发现能明显降低实验小鼠的体重。表明CNTF-D在体内具有良好的生物学活性,为下游开发奠定了基础。
|
| 关 键 词： | 睫状神经营养因子 点突变 基因表达 |
| 文章编号： | 1005-5673（2006）04-0010-04 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006-06-05 |
Construction, expression and activity analysis of recombinant human ciliary neurotrophic factor(CNTF)derivative |
| |
| ZHANG Zhen-long, XU Jing, GUO Xiu-mei,et al..Construction, expression and activity analysis of recombinant human ciliary neurotrophic factor(CNTF)derivative[J].Progress In Microbiology and Immunology,2006,34(4):10-13. |
| |
| Authors: | ZHANG Zhen-long XU Jing GUO Xiu-mei |
| |
| Institution: | ZHANG Zhen-long, XU Jing, GUO Xiu-mei, et al. |
| |
| Abstract: | Based on the natural human CNTF gene,the mutated hCNTF gene was generated by site-specific mutagenesis.The gene was inserted into E.coli and confirmed by DNA sequencing.The high expression of CNTF-D has been achieved.The expression level of recombinant protein reached to more than 40%.The purity of CNTF-D was about 95% after denaturing and purifying by Q-Sepharose HP chromatography.The biological activity of CNTF-D was measured by mouse in vivo test.The results showed that this mutant protein could obviously reduce body weight of tested mouse.It laid a foundation in development of a new type of high efficient body weight reducing drug. |
| |
| Keywords: | Ciliary neurotrophic factor(CNTF) Site-specific mutagenesis Gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
