Cell-derived sequences in the N-terminal region of the polyprotein of a cytopathogenic pestivirus |
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Authors: | Müller A Rinck G Thiel H-J Tautz N |
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Institution: | Institut für Virologie (Fachbereich Veterin?rmedizin), Justus-Liebig-Universit?t Giessen, D-35392 Giessen, Germany. |
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Abstract: | Efficient proteolytic release of nonstructural protein 3 (NS3) from the viral polyprotein is considered to be crucial for the cytopathogenicity of pestiviruses. Here we describe a novel cytopathogenic (cp) bovine viral diarrhea virus strain (BVDV CP8) with a complex insertion composed of viral and cell-derived sequences, including two fragments of the cellular J-domain protein Jiv (J-domain protein interacting with viral protein) located in the N-terminal region of the polyprotein. BVDV CP8 expresses a Jiv fusion protein of 513 amino acids in addition to a complete set of viral proteins. This protein has the capacity to induce NS2-3 cleavage in trans. Accordingly, CP8 is a representative of a novel type of cp pestivirus with a cp-specific mutation located outside of the NS2-3 gene. |
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