| 海绵共生微生物研究中的分子技术 |
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| 引用本文： | 胡叶,李志勇. 海绵共生微生物研究中的分子技术[J]. 生物技术通报, 2005, 0(6): 56-61 |
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| 作者姓名： | 胡叶 李志勇 |
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| 作者单位： | 上海交通大学生命科学技术学院海洋生物技术实验室,上海,200240 |
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| 基金项目： | 国家高新技术发展计划(863)资助(2002AA628080,2004AA628060),上海市青年科技启明星计划资助(04QMX1411),上海高校优秀青年教师后备人才计划资助 |
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| 摘 要： | 海绵共生微生物与海绵活性物质有紧密的关系,由于其大多不可培养,分子生物学技术成为海绵微生物研究的有效方法。本文重点介绍了海绵共生微生物研究中经常应用到的FISH、PCR、16S rRNA克隆测序、DGGE、RFLP等各种分子技术,对它们的特点及缺陷进行了分析与小结,希望有助于海绵共生微生物分子研究的进一步发展。
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| 关 键 词： | 分子技术 海绵 微生物 |
| 收稿时间： | 2005-06-21 |
| 修稿时间： | 2005-06-21 |
The Molecular Techniques in the Research of Sponge-associated Bateria |
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| Hu Ye,Li Zhiyong. The Molecular Techniques in the Research of Sponge-associated Bateria[J]. Biotechnology Bulletin, 2005, 0(6): 56-61 |
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| Authors: | Hu Ye Li Zhiyong |
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| Affiliation: | Marine Biotechnology Laboratory, School of Life Science and Biotechnology Shanghai Jiao Tong University, Shanghai 200240 |
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| Abstract: | Sponge-associated bacteria have special relations with sponge bioactive compounds.Because most of sponge-associated bacteria are uncultrulable,so molecular techniques as effective strategies should be applied.In this article,some molecular techniques which have been used frequently in the research of sponge-associated bacteria such as FISH,PCR,16S rRNA cloning and sequecing,DGGE and RFLP were reviewed.The advantages and disadvantages of these techniques have been mentioned.We hope this article can make for the further development in the research of sponge-associated bacteria. |
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| Keywords: | Molecular techniques Sponge Bacteria |
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