Light effects on in vitro rooting of pear cultivars of different rhizogenic ability |
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Authors: | Gianpaolo Bertazza Rita Baraldi Stefano Predieri |
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Institution: | (1) Istituto di Ecofisiologia delle Piante Arboree da Frutto, Consiglio Nazionale delle Ricerche, via Gobetti 101, 40129 Bologna, Italy |
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Abstract: | The effect of varying light regimes on in vitro rooting of microcuttings of two pear (Pyrus communis L.) cultivars was investigated. Cultures of the easy to-root Conference and the difficult-to-root Doyenne d'Hiver were incubated for 21 days with or without indole-3-butyric acid (IBA) in the medium in darkness or under continuous far-red (8 µmol m–2 s–1), blue, white or red (15 or 36 µmol m–2 s–1) light. Conference rooted without IBA when exposed to red, blue or white light while no rooting was observed under far-red light and in darkness. The high rooting efficiency under red and, by contrast, the inhibition under far-red light and darkness suggest the involvement of the phytochrome system in rhizogenesis. The addition of IBA to the culture medium enhanced root production under all light regimes in both cultivars. Red light, especially at the lower photon fluence rate, had a positive effect by increasing root extension (number × length of roots) and stimulating secondary root formation.Abbreviations IBA
Indole-3-butyric acid
- R
red light
- B
blue light
- FR
far-red light
- W
white light
- D
darkness
- Pfr
active (far-red light absorbing) form of phytochrome
- Ptot
total phytochrome
- BA
benzyl-adenine |
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Keywords: | auxin light micropropagation Pyrus communis phytochrome rhizogenesis |
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