Alginate films for delivery of root-knot nematode inoculum and evaluation of microbial interactions |
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Authors: | Rodrigo Rodriguez-Kábana Nancy Kokalis-Burelle Sebastian Kiewnick Ralf-Peter Schuster Richard A Sikora |
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Institution: | 1. Department of Plant Pathology, Auburn University, 36549-5409, Auburn, AL, USA 2. Institut für Pflanzenkrankheiten der Universit?t Bonn, Nussallee 9, D53115, Bonn 1, Germany
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Abstract: | A method was developed for utilizing alginate films to deliver inoculum into soil and evaluate microbial antagonistic activity
against nematode eggs. Eggs of Meloidogyne incognita were harvested from galled tomato roots (Lycopersicon esculentum), surface disinfested, suspended in 2% (w/v) aqueous sodium alginate, and applied to 2.5 × 5.0 cm polyvinyl chloride coated
fiberglass screens (1.5 mm2 mesh size) at a uniform thickness of 0.5 mm. The alginate solution was gelled by dipping in 0.25 M CaCl2. Films containing eggs were observed in vitro and egg development was evaluated. The number of immature eggs and eggs with
first stage juveniles declined linearly over time while the number of empty eggs shells, and hatched juveniles increased over
time, indicating that the alginate gel did not inhibit development and motility of M. incognita juveniles. In a greenhouse experiment using cucumber (Cucumis sativus) the number of galls g-1 root was correlated with the number of eggs in alginate films placed in each pot at planting. Films containing M. incognita eggs were buried in field soil containing organic amendments, incubated, removed from soil, rinsed with water, and observed.
The number of immature eggs in grids from soil amended with chitin or flax seed meal were lower than in untreated soil; percent
parasitized eggs was also greater in films from amended soil than from untreated soil. |
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Keywords: | alginate biological control Cucumis sativus Lycopersicon esculentum Meloidogyne incognita microbial ecology nematode eggs soil microbiology |
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