Chromium oxalate: A new spin label broadening agent for use with thylakoids |
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| Authors: | Steven P Berg Donna M Nesbitt |
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| Institution: | Department of Biological Sciences, University of Denver, Denver, CO 80208, U.S.A. |
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| Abstract: | Potassium tris(oxalato)chromate(III) trihydrate (chromium oxalate) has been shown to be a more useful broadening agent than potassium ferricyanide for the spin label 2,2,6,6-tetramethylpiperidine-N-oxy-4-amine (Tempamine) in thylakoid suspensions. Our data show that chromium oxalate is less permeable than ferricyanide, does not inhibit thylakoid electron transport or photophosphorylation, and is not photoreduced by thylakoids. |
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| Keywords: | Chromium oxalate Thylakoid Spin label broadening Tempamine Photophosphorylation Electron transport ESR Chloroplast |
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