Rat hypothalamic GRF elicits its biologic action in GH3 cells by interaction with VIP- preferring receptor site(s) |
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| Authors: | F N Zeytin F Reyl-Desmars T Rathbun |
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| Institution: | Laboratories for Neuroendocrinology, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California, 92037, USA |
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| Abstract: | GH3 cells can be used effectively to study the in vitro mechanism of action of GRF. In these cells, there is a time and concentration-dependent release of cAMP into the medium. Rat hypothalamic GRF, (rGRF) is 7 to 10 fold more active than human hypothalamic GRF (hGRF). VIP, a peptide which is structurally homologous to GRF, stimulates cAMP efflux in GH3 cells, with a higher affinity than hGRF or rGRF. We propose that in contradistinction to the normal rat pituitary, the stimulation of cAMP release by GRF in GH3 cells occurs via activation of VIP-preferring receptors and that GRF (rGRF in particular) behaves as a partial VIP agonist. |
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| Keywords: | GRF growth hormone releasing factor rGRF rat GRF hGRF human GRF GH growth hormone VIP vasoactive intestinal peptide MSH melanocyte stimulating hormone ACTH adrenocorticotrophic hormone DMEM Dulbecco's modified Eagle's medium |
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