Simultaneous extraction and separation of liquiritin,glycyrrhizic acid,and glabridin from licorice root with analytical and preparative chromatography |
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Authors: | Minglei Tian Hongyuan Yan Kyung Ho Row |
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Institution: | (1) Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, Incheon, 402-751, Korea |
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Abstract: | Simultaneous extraction and separation of liquiritin, glycyrrhizic acid, and glabridin from licorice were developed by liquidliquid
extraction with liquid chromatography separation. By utilizing different extraction solvents, procedures, and times, the optimum
extraction conditions were established. The extracts of licorice were separated and determined using a C18 column with a mobile phase consisting of acetonitrile-water (containing 1.0% acetic acid) with a gradient elution of 0∼10
min from 20:80 to 60:40 (v/v). Preparative columns with different packing sizes were investigated to isolate the three compounds
from the extracts of licorice. The 12 μm chromatographic column showed better separation for the three compounds from licorice.
0.29 mg/g for liquiritin, 1.43 mg/g for glycyrrhizic acid, and 0.07 mg/g for glabridin were obtained and the recoveries were
80.8, 89.7, and 72.5%, respectively. |
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Keywords: | extraction and separation liquiritin glycyrrhizic acid glabridin preparative licorice |
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