离子束介导玉米DNA导入水稻引起遗传变异的RAPD分析

本实验采用４０ 个随机引物对低能离子束介导紫玉米全ＤＮＡ 转化水稻早籼２１３ 获得的８ 个玉米稻株系基因组ＤＮＡ 进行ＲＡＰＤ检测。其中３６ 个随机引物得到扩增图谱。统计分析图谱中的各类扩增带，其中变异株系与早籼２１３ 的相似率为９１．２—９５．５％ 。差异带占总带数的８．９—１７．７％ ，说明外源ＤＮＡ 导入受体细胞引起后代基因组ＤＮＡ 的显著变异，这些变异很可能是表型及生理性状变异得以稳定遗传的物质基础。

RAPD Analysis of Hereditary and Variation of Maize Rice Developed by Ion Beam Mediated Transferring Maize DNA into Rice

Eight maize rice line, developed by low energy ion beammediated transferring maize DNA into rice EI213, have stronger root system and exhibit purple red character of maize in shoot, glumal tip and stigma. Also the photosynthetic efficiency of maize rice have increased by averaged 84% compared with that of the EI213. Genomes of the 8 maize rice, original EI213 and maize were analyzed by RAPD with 40 10mer primers. Polymorphic fingerprints were obtained from 36 primers. Different kinds of amplified bands in RAPD patterns were calculated and analyzed, the similarity of maize rice and EI213 ranged 91.295.5%. The variant bands between maize rice and EI213 counted for 8.917.7% of the total bands of each line. The result indicated that exogenous DNA introduced into rice induced remarkable variation in progeny genomes. Those hereditary changes in plant phenotype and physiology may due to the genomes variation.