Secretory expression of a heterologous nattokinase in <Emphasis Type="Italic">Lactococcus lactis</Emphasis> |
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| Authors: | Xiaobo Liang Lixin Zhang Jin Zhong Liandong Huan |
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| Institution: | (1) Center for Metabolic Engineering of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China;(2) State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China;(3) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, People’s Republic of China;(4) South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China |
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| Abstract: | Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy
to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P
nisZ and signal peptide SPUsp were used for inducible and secretory expression of nattokinase in L.
lactis. Western blotting analysis demonstrated that nattokinase was successfully expressed, and about 94% of the enzyme was secreted
to the culture. The recombinant nattokinase showed potent fibrinolytic activity, equivalent to 41.7 urokinase units per milliliter
culture. Expression and delivery of such a fibrinolytic enzyme in the food-grade vehicle L.
lactis would facilitate the widespread application of nattokinase in the control and prevention of thrombosis diseases. |
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