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pH modulation of large conductance potassium channel from adrenal chromaffin granules |
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| Authors: | Renata Hordejuk Nikolai A Lobanov Anna Kicinska Krzysztof Dolowy |
| Institution: | 1. Department of Biophysics, Agriculture University SGGW, 159 Nowoursynowska St., 02-776, Warsaw, Poland;2. Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur St., 02-093, Warsaw, Poland;3. Laboratory of Protein Chemistry, Institute of Bioorganic Chemistry, Belarus National Academy of Sciences, Kuprevicha 5/2, 220141, Minsk, Belarus;4. Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur St., 02-093, Warsaw, Poland |
| Abstract: | We report here that large conductance K+ selective channel in adrenal chromaffin granules is controlled by pH. We measured electrogenic influx of 86Rb+ into chromaffin granules prepared from bovine adrenal gland medulla. The 86Rb+ influx was inhibited by acidic pH. Purified chromaffin granule membranes were also fused with planar lipid bilayer. A potassium channel with conductance of 432±9 pS in symmetric 450 mM KCl was observed after reconstitution into lipid bilayer. The channel activity was unaffected by charybdotoxin, a blocker of the Ca2+-activated K+ channel of large conductance. It was observed that acidification to pH 6.4 cis side of the membrane lowered the channel open probability and single channel conductance. Whereas only weak influence on the single channel current amplitude and open probability were observed upon lowering of the pH at the trans side. We conclude that a pH-sensitive large conductance potassium channel operates in the chromaffin granule membrane. |
| Keywords: | intracellular chromaffin granule potassium channel adrenal chromaffin granules bilayer lipid membranes |