Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display |
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| Authors: | Hong Wang Zhenbiao Xia Yaohe Wang Chunhai Li Li Ya Sun |
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| Affiliation: | (1) Department of Tumor Molecular Biology, Institute of Basic Medical Sciences and Tai Ping Hospital, 100850 Beijing, P.R. China;(2) Institute of Radiation Medicine, 100850 Beijng, P.R. China;(3) Department of Pathology, Henal Medical University, 40052 Zhengzhou |
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| Abstract: | Methods for retrieving and reamplifying the differentially expressed cDNA bands have been modified. Direct reamplification of differentially expressed bands after cutting from a polyacrylamide gel (PAG) followed by a simple rinse and crush step has proved to be more convenient and effective than the traditional glycogen-precipitation method. Combination of 30 cycles of differential display (DD) polymerase chain reaction (PCR) and 20 cycles of standard PCR reaction also yielded higher reamplification rates. |
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| Keywords: | mRNA differential display differentially expressed cDNA bands retrieving (or recovering) and reamplifying |
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