Efficiency of pretreatments for optimal enzymatic saccharification of soybean fiber |
| |
Authors: | Karki Bishnu Maurer Devin Jung Stephanie |
| |
Institution: | a Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011-1061, USA b Center for Crops Utilization Research Center, Iowa State University, Ames, IA 50011-1061, USA |
| |
Abstract: | The effectiveness of several pretreatments high-power ultrasound, sulfuric acid (H2SO4), sodium hydroxide (NaOH), and ammonium hydroxide (NH3OH)] to enhance glucose production from insoluble fractions recovered from enzyme-assisted aqueous extraction processing of extruded full-fat soybean flakes (FFSF) was investigated. Sonication of the insoluble fraction at 144 μmpp (peak-to-peak) for 30 and 60 s did not improve the saccharification yield. The solid fractions recovered after pretreatment with H2SO4 1% (w/w), 90 °C, 1.5 h], NaOH 15% (w/w), 65 °C, 17 h], and NH3OH 15% (w/w), 65 °C, 17 h] showed significant lignin degradation, i.e., 81.9%, 71.2%, and 75.4%, respectively, when compared to the control (7.4%). NH3OH pretreatment resulted in the highest saccharification yield (63%) after 48 h of enzymatic saccharification. A treatment combining the extraction and saccharification steps and applied directly to the extruded FFSF, where oil extraction yield and saccharification yield reached 98% and 43%, respectively, was identified. |
| |
Keywords: | AC accellerase 1000 AEP aqueous extraction processing AIL acid insoluble lignin ASL acid soluble lignin db dry basis EAEP enzyme-assisted aqueous extraction processing FFSF full-fat soybean flakes FPU filter paper units LSD least significant difference NA not available ND not determined NREL national renewable energy laboratory P6L protex 6L P50FP protex 50FP S R solid remaining SLR solid-to-liquid ratio |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|