Expression and characterization of the tau subunit of phosphorylase kinase |
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| Authors: | L R Chen C J Yuan G Somasekhar P Wejksnora J E Peterson A M Myers L Graves P T Cohen E F da Cruz e Silva D J Graves |
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| Institution: | Dept. of Biochem. and Biophys., Iowa State Univ., Ames 50011. |
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| Abstract: | A cDNA encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-tau subunit protein shows enzymatic activity similar to the HPLC-purified and renatured tau subunit. |
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