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Geodermatophilus saharensis sp. nov., isolated from sand of the Saharan desert in Chad |
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| Authors: | M. C. Montero-Calasanz M. Göker G. Pötter M. Rohde C. Spröer P. Schumann A. A. Gorbushina H.-P. Klenk |
| Affiliation: | 1. Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstra?e 7B, 38124, Braunschweig, Germany 2. IFAPA-Instituto de Investigación y Formación Agraria y Pesquera, Centro Las Torres-Tomejil, Ctra. Sevilla-Cazalla de la Sierra, Km 12.2, 41200, Alcalá del Río, Sevilla, Spain 3. HZI—Helmholtz Centre for Infection Research, Inhoffenstra?e 7, 38124, Braunschweig, Germany 4. BAM, Federal Institute for Material Research and Testing, 12205, Berlin, Germany 5. Department of Biology, Chemistry and Pharmacy and Department of Earth Sciences, Free University of Berlin, 12249, Berlin, Germany |
| Abstract: | A novel Gram-positive, aerobic, actinobacterial strain, CF5/5, was isolated from soil in the Sahara desert, Chad. It grew best at 20–35 °C and at pH 6.0–8.0 and with 0–4 % (w/v) NaCl, forming black-colored colonies. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content was 75.9 mol%. The peptidoglycan contained meso-diaminopimelic acid; galactose and xylose were detected as diagnostic sugars. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol; MK-9(H4) was the dominant menaquinone. The major cellular fatty acids were: iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 95.6–98.3 % sequence similarity with the other named members of the genus Geodermatophilus. Based on the polyphasic taxonomy data, the isolate is proposed to represent a novel species, Geodermatophilus saharensis with the type strain CF5/5T = DSM 45423 = CCUG 62813 = MTCC 11416. |
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