The life cycle of Anguina agrostis: Post-embryonic growth of the second stage larva |
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Authors: | Alan F. Bird B.A. Stynes |
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Affiliation: | CSIRO, Division of Horticultural Research, G.P.O. Box 350, Adelaide, 5001, Australia |
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Abstract: | The growth and development of the freshly hatched second stage larva (FHL2) into the infective second stage “dauer” larva (DL2) has been followed under field conditions and in callus tissue culture. A comparison of this development has been made between specimens originating from widely separated geographic regions. The FHL2's from Narrogin (Western Australia) are slightly shorter than those from Murray Bridge (South Australia) and have a mean length of 543 ± 55 μm compared with 580±51 μm. However, the lengths of the DL2s from these areas are similar, having mean lengths of 841 ± 35 μm and 849 ±26 μm respectively. Furthermore stylet lengths in all these larvae are similar and are approx. 10 μm. Morphological changes associated with the transition from FHL2 to DL2 include thickening of the cuticle, a change in shape of the lateral alae associated with stretching and the synthesis of numerous lipid storage granules. Physiological changes include a marked increase in swimming activity and the ability to enter into an anhydrobiotic state. Growth from FHL2 to DL2 in Lolium multiflorum callus tissue culture took place within 9 days at 20°C. No moulting was observed and growth did not take place beyond the DL2 stage under these conditions. |
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Keywords: | seed galls rye grass lateral alae cuticle buccal stylet electron microscope callus tissue culture growth camera lucida |
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