Genetics of gliadins coded by the group 1 chromosomes in the high-quality bread wheat cultivar Neepawa |
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Authors: | T Dachkevitch R Redaelli A M Biancardi E V Metakovsky N E Pogna |
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Institution: | (1) Sezione di S. Angelo Lodigiano, Istituto Sperimentale Cerealicoltura, via Mulino 3, 20079 S. Angelo Lodigiano, Milano, Italy;(2) Sezione di Genetica Applicata, Istituto Sperimentale Cerealicoltura, via Cassia 176, 00100 Roma, Italy |
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Abstract: | Summary The inheritance and biochemical properties of gliadins controlled by the group 1 chromosomes of the high-quality bread wheat cultivar Neepawa were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contains two gliadin loci, one (Gli-B1) coding for at least six - or -gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM. Amongst the chromosome 1A gliadins, three proteins are encoded at the Gli-A1 locus whereas polypeptides N14-N15-N16 are controlled by a remote locus which recombines with Gli-A1. Six other gliadins are controlled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cultivars sharing the Gli-B1 allele of Neepawa were found to differ in the presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a reducing agent during two-dimensional sodium dodecyl sulphate polyacrylamid-gel electrophoresis, suggesting the absence of intra-chain disulphide bonds in their structure.Research supported by a grant from the Commission of the European Communities, ECLAIR programme, Contract AGRE 0052 |
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Keywords: | Gliadin loci Group 1 chromosomes Recombination Electrophoresis Triticum aestivum |
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