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Isolation and characterization of microsatellite markers in the southern emu‐wren (Stipiturus malachurus: Aves)
Authors:G S MAGUIRE  P‐J GUAY  R A MULDER
Abstract:We isolated and characterized eight novel microsatellite loci in the southern emu‐wren (Stipiturus malachurus). We used nonradioactive polymerase chain reaction (PCR)‐based techniques to screen an enriched genomic DNA library. Based on genotypes from a single population, six loci showed no evidence of null alleles and were polymorphic (allele range = 2–9, mean heterozygosity = 0.57), and one locus was sex‐linked (NA = 4). These loci were variable and had different allele size ranges in three other populations of southern emu‐wrens, and are therefore useful for determining levels of genetic diversity within and between populations of the species.
Keywords:Maluridae  microsatellite  population genetics  southern emu‐wren  Stipiturus malachurus
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