Quality indexes to assess the reliability of genotypes in studies using noninvasive sampling and multiple‐tube approach |
| |
Authors: | C. MIQUEL,E. BELLEMAIN,C. POILLOT,J. BESSI RE,A. DURAND,P. TABERLET |
| |
Affiliation: | C. MIQUEL,E. BELLEMAIN,C. POILLOT,J. BESSIÈRE,A. DURAND,P. TABERLET |
| |
Abstract: | In noninvasive studies, the intersample variance in DNA quality and quantity is large, and produces multilocus genotypes of highly variable quality. Here we propose a standardized method for testing the reliability of the genotyping procedure when using the multiple‐tube approach. The quality indexes generated will allow reliable comparisons among samples, loci, studies, and field and/or laboratory protocols. These indexes represent a powerful tool for the quality management of noninvasive studies. |
| |
Keywords: | genotyping errors microsatellites noninvasive samples quality index |
|
|