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Nine new polymorphic microsatellite loci for the amplification of archived otolith DNA of Atlantic cod,Gadus morhua L. |
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| Authors: | KLARA BJÖRG JAKOBSDÓTTIR þÓRA DÖGG JÖRUNDSDÓTTIR SIGURLAUG SKÍRNISDÓTTIR SIGRÍÐUR HJÖRLEIFSDÓTTIR GUÐMUNDUR Ó. HREGGVIÐSSON ANNA KRISTÍN DANÍELSDÓTTIR CHRISTOPHE PAMPOULIE |
| Affiliation: | 1. University of Iceland, Department of Biology, Sturlugata 7, 101 Reykjavík, Iceland, Marine Research Institute, PO Box 1390, Skúlagata 4, 121 Reykjavík, Iceland,;2. Marine Research Institute, PO Box 1390, Skúlagata 4, 121 Reykjavík, Iceland,;3. Prokaria Ltd., Gylfaflöt 5, 112 Reykjavík, Iceland |
| Abstract: | Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5–23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy–Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long‐term temporal study with DNA obtained from archived otoliths of cod. |
| Keywords: | archived DNA Atlantic cod Gadus morhua microsatellite |