| 1株产漆酶白腐真菌的筛选和鉴定 |
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| 引用本文： | 关艳丽,李莉,陈飞,郭玲玲,华霜,孙立梅.1株产漆酶白腐真菌的筛选和鉴定[J].微生物学杂志,2010,30(3):74-77. |
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| 作者姓名： | 关艳丽 李莉 陈飞 郭玲玲 华霜 孙立梅 |
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| 作者单位： | 1. 辽宁省微生物科学研究院,辽宁,朝阳,122000
2. 沈阳农业大学,土地与环境学院,辽宁,沈阳,110161 |
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| 摘 要： | 从不同的生境采集生物样品,利用Bavendamm反应反复筛选产漆酶的白腐真菌。利用真菌通用引物对ITS1/ITS4扩增菌株rDNAITS区序列,对扩增产物进行测序。测序结果在GenBank中进行同源性搜索,下载部分具有代表性种的ITS序列进行序列比对,利用软件MEGA4构建分子系统发育树,通过序列分析,并结合形态学鉴定出4220为香栓孔菌(Trametes suaveolens)。
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| 关 键 词： | 白腐真菌 变色反应 系统发育树 漆酶 |
Screening and Identification of a Laccase-producing White Rot Fungi |
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| GUAN Yan-li,LI Li,CHEN Fei,GUO Ling-ling,HUA Shuang,SUN Li-mei.Screening and Identification of a Laccase-producing White Rot Fungi[J].Journal of Microbiology,2010,30(3):74-77. |
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| Authors: | GUAN Yan-li LI Li CHEN Fei GUO Ling-ling HUA Shuang SUN Li-mei |
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| Institution: | 1. Liaoning Prov. Acad. of Microb. Sci. , Chaoyang 122000; 2. Coll. of Lend ＆ Envir. , Shenyang Agric. Uni. , Shenyang 110161) |
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| Abstract: | White rot fungi producing laccase were obtained from biological samples collected from different habitats, using Bavendamm reaction and repeatedly screening. Their rDNA ITS sequences were amplified using fungal universal primers ITS1/ITS4 to sequence the amplified products. The results of sequencing were searched in Geubank for their homology, and the part of download ITS sequence that possessed some representative species was compared with. And identified 4220 as Trametes suaveolens using a constructed molecular phylogenetic tree by sequence analysis with software MEGA4 combined with its morphology. |
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| Keywords: | white rot fungi Bavendamm reaction phylogenetic tree laccase |
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