| Folin-Ciocalteu比色法测定桦褐孔菌多酚的条件优化 |
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| 引用本文： | 张梅梅,魏志文,刘玉冰,赵艳霞,郑维发.Folin-Ciocalteu比色法测定桦褐孔菌多酚的条件优化[J].菌物学报,2011,30(2):295-304. |
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| 作者姓名： | 张梅梅 魏志文 刘玉冰 赵艳霞 郑维发 |
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| 作者单位： | 徐州师范大学江苏省药用植物生物技术重点实验室,徐州,221116 |
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| 基金项目： | 国家自然科学基金（No. 31070052）；江苏省自然科学基金（No. BK2010027 & BK2009084）；徐州师范大学自然科学基金（No. 10XLA21） |
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| 摘 要： | 以没食子酸为标准品,通过正交试验和单因素实验研究了Folin-Ciocalteu比色法测定桦褐孔菌中多酚含量的适宜条件。结果表明,优化后的显色条件为Folin-Ciocalteu试剂用量0.3mL、10%Na2CO3溶液0.6mL,25℃时避光反应30min,于750nm处测定其吸光值。多酚质量浓度在0.0032-0.0256mg/mL范围内与吸光值有良好的线性关系。根据拟合的线性回归方程对桦褐孔菌多酚进行定量测定,野生桦褐孔菌和人工培养桦褐孔菌中多酚含量最高分别为(72.05±0.08)mg/g、(52.76±0.06)mg/g(n=6)。不同加标水平的加标回收率测定实验获得的平均回收率为98.95%,RSD为1.27%。该法测定桦褐孔菌多酚具有快速、准确,稳定性强,重现性好,精密度高的特点,可应用于实际样品的测定。
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| 关 键 词： | 桦褐孔菌 多酚 Folin-Ciocalteu比色法 含量测定 |
Optimization on determination of polyphenols from Inonotus obliquus by Folin-Ciocalteu colorimetry |
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| Authors: | ZHANG Mei-Mei WEI Zhi-Wen LIU Yu-Bing ZHAO Yan-Xia and ZHENG Wei-Fa |
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| Institution: | Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China;Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China |
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| Abstract: | A quantitative method for the determination of polyphenols in sclerotia or mycelia of Inonotus obliquus was studied by orthogonal and single factor experiment with Folin-Ciocalteu reagent and standard of Gallic acid.The results showed that the content of polyphenols in I.obliquus can be well calculated according to their colorimetric absorption at 750nm by applying Folin-Ciocalteu reagent 0.3mL and 10% Na2CO3 0.6mL at 25℃ for 30min.The linear range of calibration curve was 0.0032-0.0256mg/mL for the gallic acid(r2=0.9975).Using the optimezed method,the content of polyphenols in sclerotia was determinated to be(72.05±0.08)mg/g and mycelia to be(52.76±0.06)mg/g(n=6).The average recovery was 98.95%(RSD=1.27%).Therefore,the optimized method can dectect the content of polyphenols in I.obliquus in a swift,accurate and practical way with high reproducibility. |
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| Keywords: | Inonotus obliquus polyphenol Folin-Ciocalteu colorimetry determination of content |
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