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| 巨大芽孢杆菌分子伴侣GroES和GroEL新基因的克隆及同源性分析 | |
| 引用本文： | 鲍方名,龚蕾,邵蔚蓝. 巨大芽孢杆菌分子伴侣GroES和GroEL新基因的克隆及同源性分析[J]. 中国生物化学与分子生物学报, 2007, 0(11) |
| 作者姓名： | 鲍方名 龚蕾 邵蔚蓝 |
| 作者单位： | 南京师范大学微生物工程重点实验室 南京210046 |
| 基金项目： | 江苏省高技术研究计划(No.BG2005326)~~ |
| 摘 要： | 巨大芽孢杆菌作为革兰氏阳性细菌的一种,是良好的重组蛋白的表达宿主.本研究利用PCR技术从巨大芽孢杆菌基因组克隆出一条1.9Kb的基因片段.核酸序列分析结果表明,该片段全长1984bp,包含2个ORF,分别与芽孢杆菌来源的GroES和GroEL基因有高度的相似性.氨基酸序列比对发现,GroES蛋白与枯草芽孢杆菌来源的GroES蛋白氨基酸序列同源性为91%,GroEL蛋白氨基酸序列同源性为90%. |
| 关 键 词： | 巨大芽孢杆菌 分子伴侣 GroES GroEL 基因克隆 同源性分析 |
Cloning and Sequencing of GroES and GroEL from Bacillus megaterium |
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| BAO Fang-Ming,GONG Lei,SHAO Wei-Lan. Cloning and Sequencing of GroES and GroEL from Bacillus megaterium[J]. Chinese Journal of Biochemistry and Molecular Biology, 2007, 0(11) | |
| Authors: | BAO Fang-Ming GONG Lei SHAO Wei-Lan |
| Abstract: | The sequences of GroES and GroEL of Bacillaceae bacterium were used to design a piece of primers. These primers identified a 1.9 kb clone from Bacillus megaterium genome.Complete DNA sequencing of the fragment revealed two complete open reading frames (ORFs). These ORFs exhibited a high degree of identity to the GroES and GroEL heat shock genes from other gram-positive bacteria. The order of the genes was found to be GroES-GroEL.The upstream ORF encodes a 10 kD protein which has 90% amino acid identity with GroES from Bacillus subtilis. The downstream ORF encodes a 57 kD protein which is 91% identical to GroEL from B.subtilis. |
| Keywords: | Bacillus megaterium moleeular chaperone GroES GroEL gene cloning sequencing |
| 本文献已被 CNKI 等数据库收录！ | |