ITS/CHS1靶位序列分析结合SRAP分子标记技术鉴定Nannizzia incurvata临床分离株

以转录间隔区（internal transcribed spacer,ITS）和几丁质合成酶1（chitinase synthase 1,CHS1）为鉴定靶位,结合种系进化分析将2007年至2016年分离自头癣或面癣患儿的12株石膏样小孢子菌复合体成员（现被归入Nannizzia菌属）鉴定到种水平,其中5株为Nannizzia gypsea（Microsporum gypseum）,另7株为Nannizzia incurvata（Microsporum incurvatum）。在此基础上,通过序列相关扩增多态性（SRAP）分子标记进一步证实二者在DNA多位点存在扩增多态性。本研究证实在我国湖北地区存在Nannizzia incurvata,为我国开展Nannizzia菌属的分子流行病学研究提供了实验室依据和支持。

Identification of Nannizzia incurvata based on ITS/CHS1 and SRAP molecular markers

Twelve isolates of Microsporum gypseum complex (Nannizzia species) from the children with tinea capitis or tinea faciei in our department from 2007 to 2016 were identified to species level by ITS/CHS1 sequence-based phylogenetic analysis. Five isolates were identified as Nannizzia gypsea (Microsporum gypseum), and other seven as Nannizzia incurvata (Microsporum incurvatum). The occurrence of multi-locus DNA polymorphism was confirmed in these two species by using sequence-related amplification polymorphism (SRAP) marking technique. The present study suggested the existence of Nannizzia incurvata in Hubei Province, and thus provided some experimental data and methodology for the molecular investigation of Nannizzia species in China.