Polymorphism of T-cell receptor genes among laboratory and wild mice: Diverse origins of laboratory mice |
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Authors: | Hiroaki Nobuhara Keisuke Kuida Makoto Furutani Toshihiko Shiroishi Kazuo Moriwaki Yusuke Yanagi Tomio Tada |
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Institution: | (1) Department of Maternal and Child Health, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan;(2) Department of Immunology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan;(3) Department of Cell Genetics, National Institute of Genetics, Yata 1111, 411 Mishima, Shizuoka-ken, Japan;(4) Department of Public Health, School of Medicine, Showa University, Hatanodai, Shinagawa-ku, 142 Tokyo, Japan |
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Abstract: | Southern blots of genomic DNA from 23 strains of laboratory mice and 19 individual wild mice were examined for restriction
fragment length polymorphisms in their loci encoding the T-cell receptors (Tcr): the constant regions of the α, β, and γ chains
(C
α,C
β, andC
γ) and a variable region family of the β chain (V
β8). Only a few polymorphisms were observed for each locus in the laboratory mice after using three restriction enzymes,Bam HI,Eco RI, andHind III. All the laboratory mice examined fall into one of two types for theC
α,C
β andV
β8 loci and one of three types for theC
γ. These types are found in some of the wild mice studied, indicating that they were already present in the founder mice of
laboratory mouse strains. In contrast, theTcr genes are highly polymorphic among wild mice. Analysis of the polymorphisms in these loci suggests that laboratory mice have
inherited their genes not only fromMus musculus domesticus, but also from other subspecies, and much more than previously believed from Asian subspecies. |
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Keywords: | |
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