Enzyme sensor-FIA-system for on-line monitoring of glucose, lactate and glutamine in animal cell cultures. |
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| Authors: | R Renneberg G Trott-Kriegeskorte M Lietz V J?ger M Pawlowa G Kaiser U Wollenberger F Schubert R Wagner R D Schmid |
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| Institution: | Zentralinstitut für Molekularbiologie, Berlin-Buch, F.R.G. |
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| Abstract: | Enzyme sensors for glucose, lactate and glutamine were connected via flow-injection analysis (FIA) devices to two different bioprocesses. They were used for on-line process control of perfused bioreactor systems containing mammalian cell lines producing a monoclonal antibody and recombinant interleukin-2. The biosensor system gives direct access to important process data which can be used as control parameters for long term cell cultivation systems. |
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