| Abstract: | Matney, Thomas S. (The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas), and Joan C. Suit. Synchronously dividing bacterial cultures. I. Synchrony following depletion and resupplementation of a required amino acid in Escherichia coli. J. Bacteriol. 92:960-966. 1966.-A procedure was developed for phasing large-volume cultures of Escherichia coli K-12 with regard to cell division. The method consists of permitting the bacteria to exhaust a growth-limiting supply of a required amino acid, starving the culture, resupplementing with an excess of the amino acid, and following the ensuing growth by usual counting procedures. |
