Resident CD8+ and Migratory CD103+ Dendritic Cells Control CD8 T Cell Immunity during Acute Influenza Infection |
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| Authors: | Jason Waithman Damien Zanker Kun Xiao Sara Oveissi Ben Wylie Royce Ng Lars T?gel Weisan Chen |
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| Affiliation: | 1. Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, Western Australia, Australia.; 2. Ludwig Institute for Cancer Research (Melbourne-Austin Branch), Melbourne, Victoria, Australia.; 3. School of Molecular Science, La Trobe University, Melbourne, Victoria, Australia.; Massachusetts General Hospital, United States of America, |
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| Abstract: | The identification of the specific DC subsets providing a critical role in presenting influenza antigens to naïve T cell precursors remains contentious and under considerable debate. Here we show that CD8+ T lymphocyte (TCD8+) responses are severely hampered in C57BL/6 mice deficient in the transcription factor Batf3 after intranasal challenge with influenza A virus (IAV). This transcription factor is required for the development of lymph node resident CD8+ and migratory CD103+CD11b− DCs and we found both of these subtypes could efficiently stimulate anti-IAV TCD8+. Using a similar ex vivo approach, many publications on this subject matter excluded a role for resident, non-migratory CD8+ DC. We postulate the differences reported can partially be explained by how DC are phenotyped, namely the use of MHC class II to segregate subtypes. Our results show that resident CD8+ DC upregulate this marker during IAV infection and we advise against its use when isolating DC subtypes. |
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