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Protein phosphatase 2A: identification in Oryza sativa of the gene encoding the regulatory A subunit |
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| Authors: | Yu Shuliang Lei Haiyan Chang Weizhong Söll Dieter Hong Guofan |
| Affiliation: | (1) National Center For Gene Research, Chinese Academy of Sciences, 500 Caobao Road, Shanghai, 200233, China;(2) Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, China;(3) Department of Molecular Biophysics and Biochemistry, Yale University, P.O. Box 208114, 266 Whitney Avenue, New Haven, CT 06520-8114, USA |
| Abstract: | A 2225 bp cDNA, designated RPA1, was isolated from an Oryza sativa cDNA library. Analysis revealed a 1761 bp coding sequence with 15 non-identical repeat units. The ORF encoded the A regulatory subunit of protein phosphatase 2A (PP2A-A) as ascertained by complementation of the yeast tpd3 mutant defective in this gene. The corresponding genomic DNA from a rice genome BAC library revealed that the gene contains eleven introns. The rice genome contains only a single copy of this gene as judged by Southern blot analysis. The PP2A protein is highly conserved in nature; the rice protein shows 88% amino acid identity with its counterparts in Arabidopsis or Nicotiana tabacum. |
| Keywords: | A regulatory subunit cDNA genomic sequence Oryza sativa protein phosphatase 2A |
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