Long-term stable production of monocyte-colony inhibition factor (M-CIF) from CHO microcarrier perfusion cultures |
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Authors: | Deyu Kong Reiner Gentz Junli Zhang |
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Institution: | (1) Human Genome Sciences, Inc., 9410 Key West Ave., Rockville, MD, 20850, U.S.A |
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Abstract: | Monocyte-colony inhibition factor (M-CIF) was produced in microcarrier perfusion cultures from engineered Chinese hamster
ovary (CHO) cells. Three and fifteen liter microcarrier perfusion bioreactors equipped with internal spin filters were operated
for over two months. Approximately 60 L and 300 L of culture filtrate were harvested from the 3L and 15L microcarrier perfusion
bioreactors respectively. During the perfusion operation, cell density reached 2–6 × 106 cells/ml. Importantly, stable expression of M-CIF from the CHO cells under non-selection condition was maintained at a level
of 4–10 mg/L. Specific productivity was maintained at 1.8–3.4 mg/billion cells/day. The ability of the recombinant CHO cells
to migrate from microcarrier to microcarrier under our proprietary HGS-CHO-3 medium greatly facilitated microcarrier culture
scale-up and microcarrier replenishment. Future directions for microcarrier perfusion system scale-up and process development
are highlighted.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | CHO microcarrier M-CIF perfusion spin filter stable production |
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