Identification of L-altrose in the extracellular polysaccharide from Butyrivibrio fibrisolvens strain CF3 |
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Authors: | Robert J Stack |
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Institution: | Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL, U.S.A. |
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Abstract: | Abstract Butyrivibrio fibrisolvens strain CF3, a stricyly anaerobic bacterial isolate from the ovine cecum, produces extracellular polysaccharides (EPS) when grown on a defined medium containing glucose as the carbon source. EPS were purified from culture supernatants and their monosaccharide composition was determined by two different procedures. Analysis of EPS hydrolysates by thin-layer chromatography (TLC) yielded spots coincident with standard glucose, altrose, and 1,6-anhydroaltrose. These results were corroborated by both gas-liquid chromatography (GLC) and GLC-mass spectroscopy (GLC-MS) of alditol acetates prepared from EPS hydrolysates. Purification of the altrose from EPS hydrolysates was accomplished by preparative paper and column chromatography. Polarimetry demonstrated the isolated altrose to have the L -configuration. The occurrence of this hexose in nature has not yet been reported. |
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Keywords: | Butyrivibrio fibrisolvens L-Altrose Polysaccharide extracellular |
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