A Meaningful Attempt: Applying Dielectric Barrier Discharge Plasma to Induce Polarization of Macrophages |
| |
| Authors: | Haoran Yu Wendan Cheng Chengbiao Ding Ziyu Li Wenchong Ouyang Qi Liu Zhengwei Wu Juehua Jing |
| |
| Institution: | 1. Department of Orthopaedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, China;2. Department of Rehabilitation Medicine, The Second Affiliated Hospital of Anhui Medical University, Hefei, China
School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China;3. School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China |
| |
| Abstract: | Macrophage polarization plays an important role in many macrophage-related diseases. This study was designed to preliminarily explore the effects of dielectric barrier discharge (DBD) plasma on the polarization direction and cell activity of macrophages with different phenotypes (ie, M0, M1, and M2). The M1 macrophage marker inducible nitric oxide synthase (iNOS) and M2 macrophage marker cluster of differentiation 206 (CD206) were detected by western blot (WB). The effects of DBD plasma on macrophage viability were analyzed by using a cell counting kit-8 detection kit. M0, M1, and M2 macrophages exhibited a decrease in iNOS expression and an increase in CD206 expression after the DBD plasma intervention. Additionally, the decrease in macrophage viability remained non-significant after initiating the intervention. DBD plasma can promote the transformation of M0 and M1 macrophages to M2 macrophages, and can further enhance the expression of the M2 macrophage phenotype marker CD206. Our study not only demonstrates the potential therapeutic value of DBD plasma for macrophage-related diseases, but it also provides a new direction for research to improve the treatment of macrophage-related diseases. © 2023 Bioelectromagnetics Society. |
| |
| Keywords: | DBD plasma macrophage polarization |
|
|
