Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright-Yellow 2 cells |
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Authors: | Vacca Rosa A Valenti Daniela Bobba Antonella de Pinto Maria C Merafina Riccardo S De Gara Laura Passarella Salvatore Marra Ersilia |
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Affiliation: | Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, I-70126 Bari, Italy. |
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Abstract: | To find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS-PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase-3-like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS-PCD upstream of the caspase-like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase-3-like protease activation. |
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Keywords: | PCD, programmed cell death HS-PCD, programmed cell death induced by heat shock MG132-HS cells, MG132-treated heat shocked cells ROS, reactive oxygen species cyt c, cytochrome c cAPX, cytosolic ascorbate peroxidase mGDH, mitochondrial glutamate dehydrogenase |
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