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Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright-Yellow 2 cells
Authors:Vacca Rosa A  Valenti Daniela  Bobba Antonella  de Pinto Maria C  Merafina Riccardo S  De Gara Laura  Passarella Salvatore  Marra Ersilia
Affiliation:Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, I-70126 Bari, Italy.
Abstract:To find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS-PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase-3-like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS-PCD upstream of the caspase-like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase-3-like protease activation.
Keywords:PCD, programmed cell death   HS-PCD, programmed cell death induced by heat shock   MG132-HS cells, MG132-treated heat shocked cells   ROS, reactive oxygen species   cyt c, cytochrome c   cAPX, cytosolic ascorbate peroxidase   mGDH, mitochondrial glutamate dehydrogenase
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